Journal: EBioMedicine

Article Title: Renal interstitial fibroblasts coproduce erythropoietin and renin under anaemic conditions

doi: 10.1016/j.ebiom.2021.103209

Figure Lengend Snippet: Renal interstitial cells express renin in kidneys of CKD patients, and a schematic diagram of Epo and renin production in kidneys. (a) Representative images of ISH of REN (brown) in kidney sections of patients suffering from chronic tubulointerstitial nephritis. Arrowheads and dotted circles are interstitial fibroblasts positive for REN mRNA expression and glomeruli, respectively. Red and blue arrows indicate JG cells that are positive and negative for REN mRNA expression, respectively. The scale bar is 100 μm. (b) A schematic diagram of Epo and renin production in the kidney under normal, anaemic, and injured conditions. Renal interstitial fibroblasts (yellow) maintain the systemic oxygen supply under anaemic conditions through inducing Epo and renin production in response to hypoxia and hypotension, respectively. In injured kidneys, most interstitial fibroblasts are transformed into myofibroblasts (orange), which lose the ability to produce Epo and enhance renin production.

Article Snippet: Formalin-fixed paraffin-embedded kidney sections from patients suffering from chronic tubulointerstitial nephritis were purchased from OriGene (CS700464 and CS705284 for Patient 1 and Patient 2, respectively).

Techniques: Expressing, Transformation Assay